The purpose of this study was to evaluate two different locally based regression methods (LOCAL and Local Calibration by Customized Radii Selection) and compare their performance to the classical global PLS for large NIR data. The data used in this study came from two inter-laboratory studies for wheat grain analysis organized in 2016 in the framework of the REQUASUD network. The results showed that improved predictions in terms of prediction errors can be obtained using local approaches compared to the classical global PLS. Moreover, the study highlighted clear differences between inter-laboratory studies and participating laboratories, which were even more evident when working with local procedures.